Disease vectors are prevalent and thrive well in water and this makes it inevitable to investigate the pathogenic state of every water source before it is applied for any purpose of usage. This research investigated the quality status of Otamiri River and water boreholes located in Owerri west, Imo State Nigeria. Water samples collected from the river and the selected boreholes were subjected to bacteriological, physical and chemical analyses in a laboratory sited within the locality. The results obtained indicated that the surface water is heavily adulterated, polluted and unsafe for drinking without treatment while the water from boreholes are safe for drinking without any further treatment.